The inhibitory effce t of Tongxinluo capsule on adh esion molecul es inm ice with diabetic retino pa thy through inhibiting NF-κB pathway
Abstract：Objective To investigate the effect of Tongxinluo capsule on adhesion molecules in mice with diabetic retinopathy through inhibiting NF-κB pathway.Methods KK/Upj-Ay mice were randomly divided into model group , Tongxinluo (low-dose,median-dose,high-dose) groups.C57BL/6 mice were selected as control group .The mice were given Tongxinluo intragastrically for 3 months.Then the retina barrier function was detected ,and contents of ICAM-1,VCAM-1 were determined by flow cytometry.The expression levels of ICAM-1,VCAM-1 and NF-κB in retina were detected by Real Time PCR ( qPCR) and Western Blot .Results The contents of EB in Tongxinluo median-dose and high-dose groups were obviously decreased ( P <0.01 );the serum levels of ICAM-1 and VCAM-1in Tongxinluo median-dose and high-dose groups were significantly decreased ( P <0.05);the expression levels of ICAM-1,VCAM-1 mRNA and protein in Tongxinluo groups were obviously decreased ( P <0.01);expression levels of NF-κB in Tongxinluo median-dose and high-dose groups were obviously decreased ( P <0.05),however,there were no significant differences in the expression levels of total NF-κB mRNA and protein among groups ( P >0.05).Conclusion Tongxinluo capsule can inhibit adhesion molecules in mice with diabetic retinopathy mice through inhibiting NF-κB pathway, as a result ,which can improve diabetic retinopathy .
Keywords：Tongxinluo capsulediabetic retinopathyadhesion moleculesNF-κB pathway
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 2245-2247 )