Establishment of the epileptiform discharge model in rat hippocampal neuron
Abstract：Objectvie To explore the feasibility of epileptiform discharge of rat hippocampal neurons cultured in vitro and induced by magnesium-free extracellular fluid in order to establish refractory epilepsy model in cultured cells .Mtehods The neonatal Wistar rats within 24h were used to isolate hippocampal neuron to carry out primary culture in vitro .After 12d, the hippocampal neurons were treated with magnesium free extracellular fluid for 3h,then neuronal discharge activities were recorded by whole cell patch clamping technique .Results After 12-day cell culture ,neuronal dendrites touched each other to form neural network .After 3-hour treatment by magnesium-free extracellular fluid ,the neurons produced stable discharge , however ,after 24-hour treatment with normal cell culture fluid ,the spontaneous epileptiform activity in neurons could still be observed .Conclusion The stable spontaneous epileptiform activity can be formed in hippocanlpal neurons cultured in vitro for 12d,after exposed in magnesium free extracellular fluid for 3h,which provides an ideal model for pathogenetic research about epilepsy at cell and molecular level .
Keywords：hippocampusneuronswhole-cell patch-clamp techniqueepileptiform dischargesaction potentialprimary culture
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 2093-2095 )