The role of cystathionineγlyase in the apoptosis of renal cells of neonatal rats with hypoxic-ischemic encephalopathy

doi:10.3969/j.issn.1002-7386.2014.14.001

Abstract：Objective To investigate the effect of cystathionine γlyase on apoptosis of renal cells of neonatal rats with hypoxic-ischemic encephalopathy (HIE),and to explore its action mechanism.Methods The Wistar rats (7-day age) were prepared as HIE model ,which were randomly divided into sham -operation control group and HIE group .The rates were executed at 6h,12h,24h,48h after HIE to obtain kidney tissues .The apoptosis condition of renal cells was detected by flow cytometry,the degree of inflammatory injury of renal tissue was observed by MPO ,Bax protein expression was determined by immunohistochemistry ,and the correlation between renal cell apoptosis and p-JNK signaling pathway was analyzed by Western Blot.Results In HIE group,positive apoptosis cells were increased at 6h and peaked at 24h, and the positive apoptosis cells in different time points were significantly higher than those in control group ( P >0.05).The OD value of MPO in HIE group was increased at 6h,which reached peak at 24h,and the OD value in different time points was significantly higher than that in control group ( P <0.05).The expression levels of p-JNK protein were increased at 6h and peaked at 24h.The expression levels of CSE protein in control group were significantly higher than those in HIE group ( P <0.05), furthermore,which were gradually decreased with the time going on .Conclusion The expression levels of CSE in renal cells of neonatal rats with HIE are decreased ,which has inhibitory effect on renal cell apoptosis ,which may be realized through p-JNK signaling pathway .