Expression of TNF-αand NF-кB in delaye d cerebral vasospa smaf tre SAH

doi:10.3969/j.issn.1002-7386.2014.31.004

Abstract：Objective To observe the expression of TNF-a and NF-кB in delayed cerebral vasospasm ( DCVS) after subarachnoid hemorrhage ( SAH).Methods Fifty-six Chinese rabbits were randomly divided into three groups :① control group ( n =8),the rabbits were not injected;②SAH group ( n =24),autologous arterial blood was injected into the cisterna magna at day 0 and day 2,and eight animals were killed respectively at day 4,day 7,and day 14.③Sham-operation group ( n=24),the rabbits were punctured,but not injected,and eight animals were killed respectively at day 4,day 7,and day 14.Vasospasm degree was evaluated by calculating diameter and thickness of vertebrobasilar arteries .The expression levels of TNF-αand NF-кB in delayed cerebral vasospasm were detected by immunohistochemistry and Western Blot .Results The animal models with delayed cerebral vasospasm after SAH could be established by blood perfusion into cisterna magna .The expression levels of NF-κB and TNF-αwere increased from day 4 ( P <0.01),which reached peak at day 7 ( P <0.01), and decreased to the levels of control group at day 14.Conclusion The expression levels of TNF-αand NF-кB are increased in delayed cerebral vasospasm after subarachnoid hemorrhage , and inflammatory reaction plays an important role during the process.