The effect of propofol on delayed neuronal apoptosis and Caspsa e 3 xe pression after bar in injury in rats
Abstract：Objective To explore the effect of propofol on delayed neuronal apoptosis and Caspase 3 expression after brain injury in rats in order to provide important scientific basis for clinical application of propofol .Methods Twenty-four health male Wistar rats were randomly divided into sham -operation group , physiological saline treatment group after brain injury,propofol treatment group after brain injury .Propofol was injected intraperitoneally in a dose of 100mg/kg which was equivalent to clinical dose for general anesthesia ,in which 50mg/kg propofol was administered ip 15min after brain freezing injury,and another 50mg/kg propofol was injected at 30min after the first injection.The freezing -induced brain injury models in Wistar rats were established by freezer ( pre-cold in liquid nitrogen with the temperature at -196℃)-induced brain injury in Wistar rats on the left parietal side of skull of rat for 1 min.Apoptosis of neural cells was detected by TUNEL , and the expression of Caspase 3 protein in brain of rats was detected by immunohistochemistry , moreover , neurological severity score ( NSS) was evaluated 24 hours after brain injury .Results There was a significant difference in nerve cell apoptosis between propofol treatment group and physiological saline treatment group ( P <0.05),furthermore,the apoptosis cells in both groups were significantly more than those in sham -operation group ( P <0.01).24 hours after brain freezing injury ,the expression levels of Caspase 3 protein in physiological saline treatment group were significantly higher than those in sham -operation group ( P <0.01),however,which in propofol treatment group were significantly lower than those in physiological saline treatment group ( P <0.05).After brain freezing injury,nerve function disturbance occurred in rats ,24 hours after brain injury,the NSS in propofol treatment group were significantly lower than that in physiological saline treatment group ( P <0.05).Conclusion Propofol in clinical dose for general anesthesia can reduce delayed neuronal apoptosis ,promote recovery of nerve functionafter brain injury.
Keywords：propofolbrain injuryapoptosisCaspase 3
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 1777-1780 )