Clinical significance of detection of VEGF and MVD in nephroblastoma

doi:10.3969/j.issn.1002-7386.2014.12.003

Abstract：Objective To investigate the significance of vascular endothelial growth factor ( VEGF) and microvessel density ( MVD) in pathogenesis of nephroblastoma .Methods Thirty specimens of nephroblastoma tissues including surgical resection,biopsy and serum of patients with nephroblastoma hospitalized from January 2006 to January 2013 were enrolled in the study,and these specimens were divided into I ~IV stage groups and the other two groups (Ⅰ+ⅡandⅢ+Ⅳ) according to NWTSG criteria (National Wilms’ Tumor Study Group),in which there were 4 cases at stage,5 cases atⅡstage,18 cases atⅢstage,3 cases at Ⅳstage.The expressions of VEGF and MVD in tumor tissue were detected by immunohistochemistry . The other 20 cases of nonnephroblastoma were selected as control group .Results There were no significant differences in the expression levels of VEGF and MVD in tumor tissue of children patients among different age groups and between males and females ( P >0.05).The expression levels of VEGF and MVD in tumor tissue at Ⅲ+Ⅳstage were significant higher than those at Ⅰ+Ⅱstage ( P <0.05).The expression of VEGF in tumor tissue was positively correlated with that of MVD ( r =0.672, P <0.01).Conclusion The expression of VEGF in tumor tissue can reflect the condition of MVD;the expression of VEGF,MVD in tumor tissue and serum can reflect the tumor stageing;sVEGF may be secreted from the tumors .