The construction of eukaryotic expressing plasmid PEGFP-N1-IL-2
Abstract：Objective To establish the fusion plasmid P EGFP-N1-IL-2 in order to provide basis for importing it into eukaryocyte or prokaryocyte.Methods Total RNA was extracted from the spleen of C57BL/6 mice by using TRIzol reagent, then IL-2 DNA was obtained by RT-PCR,and IL-2 DNA was cloned into PEGFP-N1 vectors,finally the fusion plasmid P EGFP-N1-IL-2 was constructed .Results The total RNA was successfully extracted from the spleen of C 57BL /6 mice,IL-2 DNA was obtained by RT-PCR,and eukaryotic expression vector -PEGFP-N1-IL-2 was successfully constructed ,the gene sequencing results showed that the sequence was completely consistent with the Genebank IL -2cDNA sequence (K02292).Conclusion The successful establishment of infusion plasmid P EGFP-N1-IL-2 provides basis for studying its expression in eukaryocyte or prokaryocyte .
Keywords：total RNAIL-2DNAplasmid PEGFP-N1-IL-2
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 1767-1769 )