Study on the expression of snail gene and protein in oral squamous cell carcinoma cell lines with different potentials of lymph node metastasis
Abstract：Objective To investigate the correlation between expression of snail gene and protein and lymph node metastasis in oral squamous cell carcinoma cell line .Methods The two cell lines of oral squamous cell carcinoma with higher or lower potential of lymph node metastasis were revived and cultured ,and the expression levels of snail gene and protein were detected by flow cytometry and RT-PCR at 12h,24h,36h,48h,60h,72h,respevtively.Results The examination results by flow cytometry showed that the positive expression rate of snail protein in the cell line with higher potential of lymph node metastasis was(48 ±4)%,which was(20 ±4)%in the cell line with lower potential of lymph node metastasis ,there was a sig-nificant difference between the two cell lines ( P <0.05).The examination results by RT-PCR showed that overexpression of snail mRNA existed in cell line with higher potential of lymph node metastasis in 6 different time points , however , low-expression of snail mRNA existed in cell line with lower potential of lymph node metastasis , there was a significant difference between the two cell lines( P <0.05).Conclusion The expression of snail mRNA and protein exists significant differences among oral squamous cell carcinoma cell lines with different potentials of lymph node metastasis ,and overexpression of snail gene and protein may be the molecular basis of lymph node metastasis in oral squamous cell carcinoma .
Keywords：oral squamous cell carcinomasnailRT-PCRflow cytometrypotential of lymph node metastasis
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 1617-1619,1620 )