Expression of Bax and Caspase-3 protein in kidney of neonatal rats with hypoxic-ischemic encephalopathy as well as the action mechanism of apoptosis in renal cells

doi:10.3969/j.issn.1002-7386.2014.11.001

Abstract：Objective To investigate the expression of Bax and Caspase-3 in kidney of neonatal rats with hypoxic-ischemic encephalopathy ( HIE) as well as the action mechanism of apoptosis in renal cells .Methods HIE models were established in seven-day-old Wistar rats ,then these rats were randomly divided into sham operation control group and HIE group.The rats were executed at 6h,12h ,24h,48h after HIE and kidney tissues were obtained .The apoptosis condition of renal cells was detected by flow cytometry ,and expression of Bax and Caspase-3 protein was determined by immunohistochemistry , the levels of TNF-α and IL-1βwere detected by ELISA , and renal cells apoptosis and P 38MAPK signaling pathway were examined by Western Blot.Results In HIE group,positive apoptosis cells were increased at 6h and reached peak at 24h, which were significantly higher than those in control group in different time points ( P <0.05).More Bax and Caspase-3 positive cells appeared at 12h in HIE group and peaked at 24h,and positive cells in different time points were significantly higher than those in control group( P <0.05).At 6h,the levels of TNF-αand IL-1βwere increased in HIE group,and TNF-αreached peak at 12h,however,IL-1βreached peak at 24h,and the levels of TNF-αand IL-1βin different time points were significantly higher than those in control group ( P <0.05).The expression levels of p-P38MAPK protein were increased at 6h in HIE group and reached the peak ,and the expression levels of p-P38MAPK protein were significantly higher than those in control group in different time points ( P <0.05).Conclusion The expression levels of Bax and Caspase-3 in renal cells of neonate rats with HIE are increased ,which suggests that the expression of Bax and Caspase-3 contributes to the apoptosis of renal cells via p-P38 MAPK signaling pathway .