An new template preparation method for rapid and sensitive PCR detection of Staphylococcus aureus in feces
FU Sulan
LIU Jingwu
SUN Wenze
Abstract：Objective To establish an new template preparation method for rapid and sensitive PCR detection of Staphylococcus aureus in feces .Methods FTA filter membrane was used to extract DNA in Staphylococcus aureus from artificially contaminated feces.Primers targeting the thermostable nuclease gene (nuc) were used to amplify a 279 bp DNA fragment which was confirmed by DNA sequencing .Results Application of FTA filter membrane could effectively extract DNA template and removed PCR inhibitive factor in specimens .In the detection of Staphylococcus aureus from artificially contaminated feces by PCR,the detection limit was 102cfu/ml,which could be finished within 6 hours.Conclusion PCR amplification using FTA filter membrane provides a faster and more sensitive method of detecting Staphylococcus aureus in feces.At the same time,which establishes a technological platform for rapid detection of foodborne pathogenic bacteria .
Keywords：PCRfecesStaphylococcus aureusFTA filter membrane
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 1295-1297 )