Effect of ulinastatin on the expression of nuclear factor-κB and interleukin-1βin rats with traumatic encephaledema
DU Yiri
DU Xuejiang
YU Jianshe
Abstract：Objective To observe the changes of expression levels of nuclear factor-κB ( NF-κB) and interleukin-1β( IL-1β) in rats with traumatic encephaledema in order to explore the protective effect and possible action mechanism of ulinastatin(UTI).Methods Thirty healthy adults SD 30 rats were randomly divided into three groups:normal control group, traumatic encephaledema group and UTI intervention group .The levels of NF-κB and IL-1βin serum and brain tissue homogenate (BTH) were detected by immunohistochemisty and enzyme-linked immunosorbent assay (ELISA) at the 8th, 12th,24th,72th hour after trauma.Results The levels of NF-κB and IL-1βin encephaledema position were significantly increased,as compared with those in control group ( P <0.01),which reached peak at the 24th hour,however,which reduced slightly at the 24th hour;there were significant differences in the changes of levels of NF-κB and IL-1βin every time point between UTI group and traumatic encephaledema group ( P <0.01).Conclusion UTI has obvious protective effect on the brain injury caused by traumatic encephaledema in rats , it may be one of the action mechanisms to inhibit the signal transduction way of NF-κB and IL-1β.
Keywords：ulinastatinprotection brainNF-κBinterleukin-1β
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 981-983 )