Effect of rhu-TNFR-Fc on the expression of TNF-α,TGF-β1 and VEGF in synovium of rats with adjuvant arthritis

doi:10.3969/j.issn.1002-7386.2014.07.002

Abstract：Objective To investigate the effect of rhu-TNFR-Fc ( etanercept ) on the expression of TNF-α, TGF-β1 and VEGF in synovium of rats with adjuvant arthritis ( AA) .Methods The adult male Wistar rats were randomly divided into 5 groups:the rats in groupⅡ~Ⅴwere injected with Freund complete adjuvant to induce the experimental animal models with AA,on the 12th day after induction ,the rats in group Ⅲwere given MTX (2.7mg/kg.w) by gavage;the rats in groupⅣand group Ⅴwere given etanercept by subcutaneous injection .After 28 days,the synovium of joint of rats was taken out to be HE stained,then synovium pathological score was calculated ,and the expression levels of TNF-α,TGF-β1 and VEGF protein in synovium of knee joint were detected by histoimmunochemistry .Results The expression levels of TNF-α,TGF-β1 and VEGF in group Ⅱ~Ⅴwere significantly higher than those in groupⅠ( P <0.05),The expression levels of TNF-αin groupⅣand group Ⅴ were obviously decreased,as compared with those in group Ⅱ ( P <0.05),however,there were no significant differences between group Ⅲand group Ⅱ( P >0.05).The expression levels of TGF-β1 and VEGF in rats'synovium tissues in group Ⅲ~Ⅴwere significantly lower than those in group Ⅱ( P <0.05).Conclusion Etanercept can obviously reduce the expression levels of TNF-α, TGF-β1 and VEGF in rats'synovium tissues , which has obvious therapeutic effect on joint inflammation of rats with AA .which may inhibit blood vessel neogenesis in local tissues .