Relationship between CETP gene polymorphism and cerebral infarction

LIU Jianqun

doi:10.3969/j.issn.1002-7386.2014.06.004

Abstract：Objective To explore the correlation between I 405V mutation of cholesteryl ester transfer protein (CETP) gene polymorphism and cerebral infarction in Han people of Hebei province by detecting CETP gene I 405V polymorphism.Methods The 350 patients with cerebral infarction (206 males and 144females) were enrolled as trial group, and 331 people without cerebral infarction or healthy subjects (190 males and 141 females) were enrolled as control group . The PCR combined with DNA direct sequencing technique was used to type the gene polymorphism at CETP I 405V(A/G)gene site,to divide into three kinds of gene types (Ⅱ、Ⅳ、ⅤⅤ) according to the occurrence condition of allele A and G .Stepwise multiple linear regression analysis was applied to explore relationship between I 405V mutation of CETP gene polymorphic site and genetic susceptibility of cerebral infarction .Meanwhile the subjects ’ blood fat levels were detected , and their smoking , drinking and family history were recorded .Results The serum levels of TC , TG, LDL-c in trial group were significantly increased,as compared with those of control group ( P <0.05),however,the serum levels of HDL-c in trial group were obviously lower than those of control group ( P <0.05);the positive rates of smoking,family history in trial group were significantly higher than those of control group ( P <0.05).The distribution frequency of II,IV,VV genotypes in trial group was 24.3%,37.4%,38.3%,respectively,however,which in control group was 50.8%,36.5%,12.7%,respectively,there was a significant difference between two groups (χ2 =8.324, P <0.01).CETP gene I405V(A/G) polymorphic site G variation allele was closely correlated to genetic susceptibility of cerebral infarction [OR=8.27,95%CI=(5.43,32.57), P<0.01)].Conclusion The I405V mutation of CETP gene may be one of the reasons of causing cerebral infarction in Han people of Hebei province .