Effects of cholecystokinin 8 on NF-κB activity in rats with hypertensive intracerebral hemorrhage
Abstract：Objective To investigate the effect of cholecystokinin-8 ( CCK-8 ) on the changes of Necular Factor Kappa B ( NF-κB) activity in rats with hypertensive intracerebral hemorrhage .Methods Seventy-two adult Sprague-Dawley rats,weighted 250 ~300 g, were randomly divided into three groups: control group , cerebral hemorrhage group and CCK-8 treatment group.The activity of NF-κB was detected by electrophoretic mobility shift assay ( EMSA) and the expression of NF-κB in cells was detected by immunohistochemical methods ,and water content in brain tissue of injury part was measured . Results The activity of NF-κB and brain water content in cerebral hemorrhage group were significantly increased , as compared with those in control group and CCK-8 group ( P <0.05).Conclusion The expression levels of NF-κB are obviously increased immediately after intracerebral hemorrhage ,and CCK-8 can inhibit effectively the activity of NF-κB,which relieves the damage of secondary inflammatory reaction induced by NF-κB after intracerebral hemorrhage .
Keywords：NF-κBcerebral hemorrhageCCK-8inflammation
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 812-815 )