Effect of compound doxycycline sustained-release gel on IL-4 and TNF-αlevels in gingival crevicular fluid in rats with experimental periodontitis

doi:10.3969/j.issn.1002-7386.2014.06.002

Abstract：Objective To observe the effect of compound doxycycline sustained-release gel on gingival index (GI), depth of pocket ( PD ) , attachment loss ( AL ) , IL-4 and TNF-αlevels in gingival crevicular fluid ( GCF ) of rats with experimental periodontitis .Methods Forty -two Wistar rats were randomly divided into 3 groups: normal control group (group N),positive control group (group C) and trial group (group T),with 14 rats in each group.After periodontitis models were established,the rats in group C were treated by minocycline ointment ,however,the rats in group T were treated by compound doxycycline sustained release gel .The rats in both groups accepted treatment once a week for 4 weeks.The GI,PD, AL,IL-4 and TNF-αlevels were detected and recorded before and after treatment .Results After treatment,GI,PD,AL in group C and group T were significantly decreased ( P <0.05).IL-4 levels in GCF of rats with periodontitis were significantly lower than those in group N ( P <0.05),however,IL-4 levels were significantly increased after treatment ( P <0.05).After treatment,IL-4 levels in group T were obviously higher than those in group C ( P <0.05).TNF-αlevels in GCF of rats with periodontitis were significantly higher than those of group N ,however ,TNF-αlevels were significantly decreased after treatment ( P <0.05).There were no significant differences in TNF-αlevels between group T and group C after treatment ( P >0.05).Conclusion Compound doxycycline sustained-release gel can obviously improve clinical symptoms of periodontitis , which can obviously decrease TNF-αlevels and increase IL-4 levels in GCF .