Effect of captopril on the expression levels of serum TNF-αin rats with pressure overload

doi:10.3969/j.issn.1002-73862.014.05.008

Abstract：Objective To observe the effect of captopril on the expression levels of serum TNF-αin rats with pressure overload.Methods The animal models with pressure overload myocardial fibrosis were established by abdominal aorta constriction in Sprague-Dawley ( SD ) rats.The rats were randomly divided into three groups :shamo-peration group , model group and captopril group .After 4 weeks, the models were successfully established and the rats were given drugs , with a treatmentcourse of 4 weeks.After 8 weeks the heart mass index and left ventricular mass index were measured ,left ventricular myocardial pathological changes were observed by means of HE staining , left ventricular myocardial collagen forms were observed by means of Masson staining ,and image analysis collagen volume fraction ( CVF)and perivascular collagen area ( PVCA) were measured by image analysis ,and the levels of serum TNF-αwere detected by ELISA .Resu lts As compared with those in sham-operation group ,the heart mass index and left ventricular mass index were significantly increased in model group ( P <0.01);as compared with those in model group,which in captopril group were significantly decreased ( P <0.01).As compared with those in sham-operation group,CVF and PVCA in model group were significantly increased ( P <0.01); as compared with those in model group ,which in captopril group were significantly decreased ( P <0.01).As compared with those in sham-operation group , the serum levels of TNF-αwere obviously increased in model group ( P <00.1);as compared with those in model group ,which in captopril group were significantly decreased ( P <0.01).Conclusion The captopril has the effect of regressing myocardial fibrosis.