Establishment of experimental animal model of maxillary sagittal expansion

doi:10.3969/j.issn.1002-7386.2014.05.007

Abstract：Objective To establish experimental animal model of maxillary sagittal expansion in New Zealand white rabbits so as to provide experiment data for clinic and scientific research .Methods The 15 New Zealand white rabbits ( body weight:1.5~1.7kg,8-week age) were randomly divided into experimental group ( n =8) and control group ( n =7).The rabbits in experimental group were placed on the expansion appliance ,inserting into the diastema between the first and second molars,however,the rabbits in control group were not treated .The rabbits in experimental group were intravenously injected tetracycline hydrochloride 25 mg/kg via ear marginal veins .After 2-week initiative expansion ,the osteoblasts in upper jaw pars palatalis were calculated , respevtively , and tetracycline hydrochloride fluorescence integral optical density calculation was performed.Results HE staining showed that the osteoblast proliferation in upper jaw pars palatalis was active , with girdle-shaped aggregation,furthermore,the osteoblasts were significantly more than those in control group ( P <0.05). Meanwhile tetracycline hydrochloride fluorescence integral optical density analysis showed that fluorescence labeling broad bands along the stretch direction in experimental group were significantly more than those in control group ,so were the intensity (P <0.05).Conclusion The experimental animal model of maxillary sagittal expansion in New Zealand white rabbits can objectively reflect bone remodeling ,with the advantages of easy operation and good repeatability .