Effect of Xuebijing injection on MMP-9 levels and its protection effect on lungs of rats with sepsis

doi:10.3969/j.issn.1002-7386.2014.05.001

Abstract：Objective To investigate the changes of serum levels of MMP-9 and the pathological changes of lung tissue in rats with sepsis induced by CLP ,and to explore the protection effect and action mechanism of Xuebijing injection . Methods Forty five male SD rats were randomly divided into 3 groups: blank group,NS group and Xuebijing group .Serum MMP-9 levels and the ratio of lung wet/dry weight were determined .The pathologic changes of lung were observed by means of light microscopy and transmission electron microscopy .Results As compared with that in blank group ,the ratio of lung wet/dry weight in NS group and Xuebijing group was significantly increased ( P <0.01),however,as compared with that in NS group,the ratio of lung wet/dry weight in Xuebijing group was significantly decreased ( P <0.01).The serum levels of MMP-9 in NS group and Xuebijing group were significantly higher than those in blank group ( P <0.01).As compared with those in NS group,the serum levels of MMP-9 in Xuebijing group was significantly decreased ( P <0.01).The lung tissue structures and vascular endothelial cell structures in blank group were basically normal ,however ,obvious inflammatory reaction and vascular endothelial cell structure injury were found in NS group , which in Xuebijing group were obviously relieved , however,which were severer than those in blank group .Conclusion Xuebijing injection can reduce MMP-9 levels, as a result,which can relieve lung vascular endothelial cell injury and improve pulmonary vasopermeability ,so Xuebijing injection has certain protection effect on lungs .