The changes of P-selectin and tissue factor in hemorrhagic fever with renal syndrome and the correlation between the changes and blood coagulation function disorder

doi:10.3969/j.issn.1002-7386.2014.04.002

Abstract：Objective To investigate the changes of Ps-electin ( P-sel) and tissue factor ( TF) in hemorrhagic fever with renal syndrome ( HFRS) and the correlation between the changes and blood coagulation function disorder ,so as to direct clinical treatment .Methods Twenty -seven patients with HFRS were divided into two groups according to the severity of illness,mild case group ( n =13) and severe case group ( n =14),and 20 healthy subjects were served as control group .The levels of plasma P-sel and TF were detected by enzyme-linked immunosorbent assay (ELISA),coagulogram was measured by immunoturbidimetry,blood urea nitrogen (BUN) and creatinine (Cr) were detected by automatic biochemical analyzer ,and platelet parameters were determined by automatic blood cell classification analyzer for both groups .Results In pyretogenic stage of HFRS,the plasma levels of TF in severe case group were significantly higher than those of mild case group ( P <0.01).The plasma levels of P-sel were positively correlated to those of polymorphonuclear neutrophils (PMN) and monocytes (mo) ( r =0.661,0.513, P <0.01).The change curve of TF was basically coincident with that of APTT ,INR,D-D,BUN, however,which was opposite to that of Fn ,platelet.The correlation analysis showed that the plasma levels of TF were positively correlated to those of APTT,INR,D-D,BUN ( r =0.436,0.351,0.386,0.362,respectively, P <0.05),however,which were negatively correlated to those of Pt ( r =-0.405, P <0.01).Conclusion The plasma P-sel plays an important role in promoting the interaction between white blood cell and endothelial cells .The increase of TF expression is the important reason of causing clotting mechanism disorder and resulting in renal function lesion .