The influence of TGFβon the expression of TLR2 regulated by AngⅡ in human fibrobal st
Abstract：Objective To investigate the effect of transforming growth factor beta ( TGF-β) on the expression of toll like receptor 2 ( TLR2 ) regulated by angiotensinⅡ( AngⅡ) in human fibroblast ( HT-1080 ) .Metho ds The cultured fibroblasts at logarithmic growth phase were divided into 5 groups:blank control group,negative control group,AngⅡgroup, TGF-βgroup,TGF-βinhibitor ( decorin )+AngⅡgroup.The expression levels of TLR2 mRNA were detected by RT-PCR in all the groups.The results were statistically analyzed .Results After treating HT-1080 with AngⅡ,the expression levels of TLR2 were significantly higher than those in blank control group ( P <0.05).The low-dose TGF-β(0.1,0.01 μg/L) increased the expression levels of TLR2, which were significantly higher than those in blank control group , however, the high-dose TGF-β(1.0,10μg/L) decreased the expression levels of TLR 2,which were significantly lower than those in blank control group ( P <0.05).The expression levels in TGF-βinhibitor (decorin )+AngⅡgroup were significantly higher than those in blank control group .Conclusion AngⅡcan up-regulate the expression levels of TLR 2.The high-dose TGF-βcan inhibit the expression of TLR2,however,low -dose TGF-βcan promote the expression of TLR2.The low -dose TGF-βin coordination with AngⅡcan increase the expression levels of TLR 2 in HT-1080 .
Keywords：fibroblastangiotensin Ⅱtransforming growth factor-βTLR2
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 485-487 )