Significance KCTD15 gene polymorphism and its correlation withg ene tic susceptibility to coronaryh eart disease

doi:10.3969/j.issn.1002-7386.2014.03.002

Abstract：Objective To explore the significance of rs 11084753 site polymorphism of potassium channel tetramerization domain containing 15 (KCTD15) gene and its relationship with genetic susceptibility to coronary heart disease (CHD).Methods The 426 patients with CHD including 216 males and 210 females were enrolled as case group ,and the other 436 healthy subjects were enrolled as control group .The rs11084753 site polymorphism of KCTD15 was detected by PCR-RFLP and DNA sequencing technology .All the tested subjects were divided into 3 groups (AA,AG,GG) according to the genotypes of rs11084753 site.The levels of serum lipids in both groups were detected simultaneously.The stepwise multiple linear regression analysis was used to analyze the correlation between rs 11084753 site polymorphism of KCTD15 gene and genetic susceptibility to coronary heart disease .Results As compared with those in control group ,the serum levels of TC,TG, LDL-C were significantly increased in case group ,however,the levels of HDL-C were obviously decreased ( P <0.05).The proportion of smoking,drinking,family history in case group was significantly higher than that of control group ( P <0.05). The distribution frequency of AA,AG,GG genotypes in case group was 52.4%,32.6%,15.0%,respectively,however,which was 76.1%,18.4%,5.5%,respectively in control group,there was a significant difference between two groups ( P <0.01). The stepwise multiple linear regression analysis showed that genotype A /G of rs11084753 site was related to genetic susceptibility to CHD (OR=5.94,95%CI=3.02, P <0.01).Conclusion The rs11084753 site polymorphism (A/G) of KCTD15 gene is related to genetic susceptibility to CHD of Han people in Hebei province .