Effect of tanshinone ⅡA on expression of myocardial NF-κB in rats with pressure overload

doi:10.3969/j.issn.1002-7386.2014.02.006

Abstract：Objective To observe the effect of tanshinone ⅡA ( Tan Ⅱ A) on expression of myocardial NF-κB in rats with pressure overload , and to explore its protective effects on myocardial fibrosis .Methods The pressure overload myocardial fibrosis models were established by abdominal aorta constriction in Sprague -Dawley ( SD ) rats, these rats were randomly divided into four groups:sham-operation group,model group,Tan Ⅱ A group (20mg· kg-1 · d-1 ) and positive control group (captopril 100mg· kg-1· d-1),with 8 rats in each group.4 weeks after the operation,the animal models were successfully established,then the rats were given drugs,with a course of 4 weeks.After 8 weeks,left ventricular mass index, myocardial tissue pathological changes ,myocardial hydroxyproline content ,myocardial NF-κB p65 expression were detected in rats of the four groups .Results As compared with those in sham -operation group , cardiac mass index and left ventricular mass index were significantly increased in model group ( P <0.01); as compared with those in model group ,these indexes were obviously decreased in Tan Ⅱ A group ( P <0.01).As compared with that in sham -operation group,myocardial hydroxyproline content was significantly increased in model group ( P <0.01),however,as compared with that in model group,myocardial hydroxyproline content was obviously decreased in Tan ⅡA group ( P <0.01).As compared with those in sham-operation group ,NF-κB p65 protein expression levels in myocardial tissues were significantly increased in model group ( P <0.01),however,as compared with those in model group ,which were significantly decreased in Tan Ⅱ A group ( P <0.01).Conclusion Tan Ⅱ A can inhibit cardiac hypertrophy ,relieve myocardial fibrosis,and the effect may be related to down-regulation of the expression of NF-κB.