Study on the effect of pirarubicin in radiosensitization of osteosarcoma cells in vitro

doi:10.3969/j.issn.1002-7386.2014.02.004

Abstract：Objective To investigate the effect of pirarubicin in radiosensitization of human osteosarcoma cells (MG-63) in vitro.Methods MTT was used to measure the inhibitory effect of pirarubicin on MG -63cells at exponential growth phase to determine IC 10 as drug concentration of experiment .After MG-63 were treated with IC10 concentration pirarubicin for 24 hours,the cells were given different doses (0,2,4,6,8Gy) of irradiation as well as IC10 concentration paclitaxel for different time points (12h,24h,36h),finally the cell survival fraction (SF) was detected by clone formation analysis method,radiosensitization ratio was calculated by fitting cell survival curve ,and the changes of cell survival rate of MG-63 treated by pirarubicin in different time points (12h,24h,36 h) were analyzed.Results After 24-hour pirarubicin administration,the cell inhibition rate was gradually increased ( P <0.01),and the drug toxicity was dose -dependent,with IC10 concentration being 0.004μg/ml.According to D0,Dq and SF2 value,the sensitivity enhancement ratio ( SER) of IC10 concentration pirarubicin was 1.49,1.06 and 1.04,respectively,theα/βratio of MG-63 cells was 2.23,after treated with IC10 concentration pirarubicin,which was 0.27.After treated with IC10 concentration pirarubicin,MG-63 cells received irradiation. The cell survival fraction was gradually decreased with the time prolongation ( P <0.01).Conclusion Pirarubicin has radiosensitizing effect on MG-63 cells,and the effect is positively correlated to effect time of drug within a certain time .