Effect of KLF4 overexpression on palmitate-induced insulin resistance in L6 skeleton muscle cells of rats
ZHANG Zhe
WANG Chao
Abstract：Objective To investigate the effect of Krüppel-like factor 4 (KLF4) overexpression on palmitate-induced insulin resistance in L6 skeleton muscle cells of rats .Methods The insulin resistance models in L6 skeleton muscle cells of rats were induced by palmitic acid , which were randomly divided into two groups , control group and experiment group .The differentiated L6 skeleton muscle cells in control group were transfected with adenovirus empty vector (Ad),however,which in experiment group were transfected with KLF4 adenovirus expression vector (Ad-KLF4).The expression levels of KLF4,insulin receptor ( IR) and glucose transporter type 4 ( GLUT4 ) in both groups were detected by Real-time PCR and Western Blot . Results As compared to those in control group ,the expression levels of KLF4,IR and GLUT4 in experiment group were significantly down-regulated( P <0.05),and the intaking amount for glucose was obviously decreased ( P <0.05).The overexpression of KLF4 could enhance the sensitivities of the cells to insulin ,and could up-regulate the expression levels of IR and GLUT4.Conclus ion KLF4 overexpression can up-regulate the expression levels of IR and GLUT 4, and can improve insulin sensitivities of L6 cells effectively .
Keywords：Krüppel-like factor 4gene transfectionskeletal muscle cellinsulin resistance
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 165-167 )