Protective effects of catalpol on PC12 cell injury induced by oxygen-glucose deprivation
WANG Ying
LIU Yugang
CHEN Zhanfa
Abstract：Objective To investigate the protective effects of catalpol on PC 12 cells damage induced by oxygen-glucose deprivation .Methods PC12 cells were pretreated with catalpol , then exposed to sodium hyposulfite to establish cell injury model .The cell survival rates were detected by MTT assay .The activities of LDH ,SOD,GSH-Px and MDA levels were detected by colorimetry ,and free Ca2+concentration within cell was detected by fluorescence probe .Results As compared with model group ,catalpol could enhance significantly survival rate of PC12 cell in a dose-dependent manner ( P <0.01),and could reduce the efflux of LDH from damaged cell and increase the activities of SOD ,GSH-Px,however,which could decrease MDA levels and intracellular Ca2+concentration ( P <0.05 or P <0.01).Conclusion Catalpol can protect PC12 cells from oxygen glucose deprivation injury , and its action mechanism may be related with removing free radical and inhibiting calcium overload .
Keywords：catalpolPC12 cellsoxygen-glucose deprivationCa2+
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 14-16 )