Biological behaviors and clinical significance of expression of THBS4 protein in gastric cancertis use in plateaua rea

doi:10.3969/j.issn.1002-7386.2014.01.003

Abstract：Objective To investigate the biological behaviors and clinical significance of expression of t hrombospondin4 ( THBS4 ) in gastric cancer tissues at plateau region in order to provide theoretical basis for prognosis evaluation of gastric cancer in plateau area .Mte hods The expression levels of THBS 4 protein in 60 gastric cancer specimens in plateau area were detected by RT-PCR and S-P.Results RT-PCR results showed that there were 5 cases that expressed THBS4 mRNA among 10 cases of well differentiated gastric cancers and there were 24 cases that expressed THBS 4 mRNA among 29 cases of poorly differentiated gastric cancers , there was an obvious difference in the expression of THBS 4 mRNA between well differentiated gastric cancers and moderately ,poorly differentiated gastric cancers .The expression levels of THBS4 mRNA in moderately , poorly differentiated gastric cancers were significantly higher than those in well differentiated gastric cancers ( P <0.05).The expression of THBS4mRNA was correlated to lymphatic metastasis ,however,which was not related to clinical stage.Immunohistochemistry (SP) results showed that there were 21 cases that expressed THBS4 protein among 29 cases of poorly differentiated gastric cancers and there were 8 cases that expressed THBS 4 protein in moderately differentiated gastric cancers ,which were higher than that ( 4 cases ) in well differentiated gastric cancers , and there was an significant difference in the expression of THBS4 between well differentiated gastric cancers and moderately ,poorly differentiated gastric cancers ( P <0.05).The positive rates of THBS4 expression in moderately,poorly differentiated gastric cancers were high than those in well differentiated gastric cancers .The expression of THBS4 gene in plateau area was correlated to differentiation degree and lymphatic metastasis of gastric cancers .Conclusion THBS4 gene expression can be used as the index for evaluating gastric cancer cell differentiation ,which plays an important role in prognosis evaluation of gastric cancer.