Effect of small int erfering RNA-induced CXCR4 sli ence gene on peritoneal metastasis of ovarian cancer in rats
YAN Xia
YAN Feng
ZHANG Jing
Abstract：Objective To observe the therapeutic effect of CXC chemokine receptor-4(CXCR4)-targeted therapy on ovarian cancer .Methods DMBA cotton thread was embedded into the ovaries of Wister rats to induce ovarian cancer model .Then 60 rat models with ovarian cancer were randomly divided into experimental group and control group by means of random digits table .The rats in experimental group were treated by gene targeted therapy,however,the rats in control group were treated by negative therapy .After siRNA silencing ovarian cancer CXCR4 gene,the expression of CXCR4protein ,its effect on cell proliferation of ovarian cancer ,peritoneal cavity metastasis after gene targeted therapy were observed ,and the survival condition of rats was evaluated .Results After treatment the CXCR4 levels in ascites of experimental group were significantly decreased ( P <0.05), however,the CXCR4 levels in ascites of control group were significantly increased ( P <0.05).There were no significant differences in the CXCR 4 levels in ascites before treatment between experimental group and control group ( P >0.05),however,there were significant differences in the CXCR4 levels in ascites after treatment between experimental group and control group ( P <0.05).As compared with that in control group,the ascites extraction volume in experimental group was obviously decreased ( P <0.05).Conclusion Application of gene targeted therapy via siRNA-induced CXCR4 silence gene is effective in inhibiting peritoneal cavity metastasis of ovarian cancer,with better prognosis .
Keywords：ovarian cancerperitoneal cavity metastasisgene targeted therapy
Publication Date：2013-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 3701-3703 )