Effects of propofol on inflammatory response induced by myocard ium ischemia/reperfusion in diabetic rats

doi:10.3969/j.issn.1002-7386.2013.24.001

Abstract：Objective To explore the molecular mechanisms of propofol in cardioprotection by means of ischemia /reperfusion injury model in diabetic rats .Methods A total of 100 SD rats were randomly divided into two groups:non-diabetes group ( n =24) and diabetes group ( n =76).Finally,60 diabetic rat models were successfully established .The rats in non-diabetes group were randomly redivided into sham-operation group (Ⅰ) , I/R group(Ⅱ),then the rats in diabetes group were randomly redivided into sham-operation group (Ⅲ),I/R group (Ⅳ) and propofol 3,6,12mg· kg-1 · h-1 (Ⅴ,Ⅵ,Ⅶ) groups,with 12 rats in each group.Heart rate (HR),mean arterial blood pressure (MAP) and blood pressure/heart rate index (PRI) were recorded during occlusion and at the end of reperfusion ,respectively.The concentrations of TNF-α,IL-6,in serum and myocardium were detected by ELISA . The translocation of NF-κB in the cardiomyocytes was determined by immunohistochemistry ,and the expression levels of NF-κB were detected by Western Blot .Results As compared with those in groupⅠ,before ischemia the cardiac function parameters ( HR,MAP,PRI) were obviously decreased in group Ⅲ( P <0.05 or <0.01).However the concentrations of TNF-α,IL-6 in serum and myocardium,the expression levels of TNF-α,IL-6 mRNA in myocardium of groupⅣwere significantly higher than those of groupⅡ( P <0.05 or <0.01).The expression levels of NF-κB in nuclei in group Ⅳwere markedly higher than those in group Ⅲ( P <0.01).As compared with those in group Ⅳ,administration of propofol at 6,12mg· kg-1 · h-1 significantly inhibited the NF-κB to translocate into nucleus and decreased the expression of NF-κB in the nuclei (P <0.05),and reduced the concentrations of TNF-α,IL-6 in serum and myocardium, respectively ( P <0.05 or <0.01).Conclusion Propofol can markedly inhibit NF-κB activation in myocardium after I/R in diabetic rats,which can also increase the expression levels of TNF-αand IL-6,as a result,which can obviously improve cardiac function.