Relationship between XspI site polymorphism of LDL-R gene and serum Hs-CRP in patients with hypercho lesterolemia
Abstract：Objective To investigate the correlation between Xsp I site polymorphism of exon 4 in low-density lipoprotein receptor (LDL-R) gene and serum high-sensitive C-reactive protein (Hs-CRP) levels in patients with hypercholesterolemia and its significance .Methods Xsp I site polymorphism of exon 4 in LDL-R gene was detected by PCR-RFLP and DNA sequencing in 1,250 patients with hypercholesterolemia and 1,200 healthy subjects .All the subjects were divided into three groups according to the genotypes of XspI site :X+X+group,X+X-group and X -X-group.The levels of serum Hs-CRP were detected by Vlin-Integral Nephelometry . The levels of serum lipids were detected simultaneously .The relationship between Hs -CRP levels and genotype of XspI was analyzed .Results The serum levels of Hs-CRP in patients were significantly higher than those of healthy subjects,however,the levels of HDL-c were significantly lower than those of controls ( P <0.05).The level of Hs-CRP were increased in the order of genotype X -X-,X+X-and X+X+,with statistical significance among the three groups ( P <0.05).Stepwise multiple linear regression analysis showed that total cholesterol affected the levels of serum Hs-CRP,and the genotype X +X+of XspI site was the independent factor of affecting serum Hs-CRP levels.Conclusion The serum levels of Hs-CRP are increased in patients with hypercholesterolemia,so are in X +X+of LDL-R gene,moreover,Xsp I site polymorphism of exon 4 in LDL-R gene can aggravate hypercholesterolemia by affecting the levels of Hs-CRP.
Keywords：hypercholesterolemialow-density lipoprotein receptorgene polymorphismhigh-sensitive C-reactive protein
Publication Date：2013-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 3528-3530 )