Preparation of sacculus injury model in abdominal aorta of rabbits

doi:10.3969/j.issn.1002-7386.2013.22.006

Abstract：Objective To investigate the preparation method of sacculus injury model in abdominal aorta of rabbits.Methods 30 New Zealand rabbits were randomly divided into 3 groups:2.5mm sacculus group ( n =10),3.5mm sacculus group ( n =10) and control group ( n =10).Except for sham-operation group,the animal models of sacculus injury in abdominal aorta of rabbits were established by 2.5mm or 3.5mm sacculus injury .The time of making models , blood vessel average stenosis extent , average thickness of neogenesis intima and tunica media were detected and compared after 4-week feeding .Results The operation time in sham-operation group was (16 ±3)min,which was significantly shorter than (29 ±4) min in 2.5mm sacculus group and (35 ±6) min in 3.5mm sacculus group ( P <0.05).As compared with that in sham-operation group,the blood vessel average stenosis extent in 2.5mm sacculus group or 3.5mm sacculus group was increased by (22.1 ±3.5)% and (32.5 ±5.8)%,respectively ( P <0.05),which in 3.5mm sacculus group was severer than that in 2.5mm sacculus group,moreover,which in 2.5mm sacculus group was severer than that in sham-operation group.The thickness of intima in 3.5mm sacculus group was(0.07 ±0.03)mm,which was significantly greater than (0.03 ±0.02)mm in 2.5mm sacculus group and (0.02 ±0.03) mm in sham-operation group ( P <0.05).The thickness of tunica media in 3.5mm sacculus group was(0.16 ±0.03)mm,which was significantly greater than (0.12 ±0.02)mm in sham-operation group ( P <0.05),however,there was no significant difference in the thickness of tunica media between 3.5mm sacculus group and 2.5mm sacculus group ( P >0.05).Conclusion The 3.5mm sacculus catheter is superior to 2.5mm sacculus catheter in making the animal model with endangium injury .