Effect of sCD40L on the secretion of tumor necrosis factor-αin human umbilical vein endothelial cells
Abstract：Objective To investigate the effect of sCD 40 L on the secretion of tumor necrosis factor-α( TNF-α) in human umbilical vein endothelial cells ( HUVEC ) .Methods The HUVECs were cultured in different groups,and HUVECs were stimulated by r-CD40L at different concentrations (0,10,20,30,40 μg/L) and in different time points (0,10,20,30,40 min),then the concentrations of TNF-αin supernatant were detected by enzyme-linked immunosorbent assay (ELISA ) .Results The levels of TNF-αsecreted by HUVEC in supernatant were the highest at 20 minutes after stimulation by r-CD40L (20 μg/L), there were significant differences among the 5 different time groups ( P <0.01).With the increase of concentration of r-CD40L,the levels TNF-αsecreted by HUVEC in supernatant were increased in a dose -dependent manner , there were significant differences among the 5 different dose groups ( P <0.01).Conclusion The levels of TNF-αsecreted by HUVEC may reach peak at early time after r-CD40L stimulation, moreover, which is in a concentration -dependent manner .
Keywords：eclampsismhuman umbilical vein endothelial cellssCD40Ltumor necrosis factor-α
Publication Date：2013-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 3205-3207 )