Effect and significance of simvastatin pretreatment on ICAM-1 expression in myocardial cells of rats with sepsis
ZHANG Litao
YU Zhibo
ZHAO Heling
Abstract：Objective To observe the expressions of ICAM-1 in myocardial cells of rats with sepsis ,and to explore the effect and significance of simvastatin pretreatment on ICAM-1 expression .Methods Sixty female Wistar rats were randomly divided into three groups:control group ( n =20 ) , sepsis group ( n =20 ) and simvastatin group ( n =20 ) .The animal models with sepsis were established by cecal ligation and puncture (CLP).The rats in simvastatin group were treated with simvastatin ,20mg/kg,once a day for two weeks before CLP.After teo weeks the rats both simvastatin group and sepsis group underwent CLP ,then 48h after operation the myocardium tissues of rats in three groups were collected to be made paraffin section , and the expressions of ICAM-1 protein in myocardial cells were detected by immunohistochemistry ( IH ) staining.Results Immunohistochemistry staining showed that the expression levels of ICAM-1 in sepsis group were significantly higher than those in control group at 48h after CLP ( P <0.05),however,which in simvastatin group were significantly decreased,as ompared with those in sepsis group ( P <0.05),and there were significant differences in expression levels of ICAM-1 between simvastatin group and control group ( P <0.05 ).Conclusion Simvastatin can attenuate the expression of ICAM-1 in myocardial cells of rats with sepsis ,as a result,which can partially improve heart function during sepsis .
Keywords：sepsissimvastatinintercellular adhesion molecule-1immunohistochemistrystaining
Publication Date：2013-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 2896-2898 )