Study on the effect and action mechanism of panax notoginseng saponins -Rg1,Rb1 on hepatic fibrosis in rats

WU Fan; LIU Pengnian; MA Yuqian

doi:10.3969/j.issn.1002-7386.2013.18.003

Abstract：Objective To investigate the protective effect panax notoginseng saponins -Rg1, Rb1 on mitochondria of rats with hepatic fibrosis .Methods The rats were randomly divided into four groups , control group,model group,Rg1 group and Rb1group.The rat models with hepatic fibrosis were established by giving 5%CCI4 solution via gavage , then hepatic morphological changes were observed by transmission electron microscope and light microscope;procollagen type Ⅲ ( PCⅢ) levels were detected by radio-immuno assay;secretory phospholipase A2 (sPLA2) activity was tested by labeled E.coli membrane with [3H] oleic acid.The changes of mitochondrial membrane fluidity were detected by fluorescent polarization;MDA content,activities of SOD,Ca2+-ATPase and total ATPase were determined by spectrophotography;activities of respiratory chain complex Ⅰ,Ⅱ,Ⅲ,Ⅳ were detected by ultraviolet spectrophotometry .Results Transmission electron microscope showed that collagen fibrils and rough bunch of collagen fibrils precipitation were found in control group .The results of HE staining and Masson collagen staining slices demonstrated that there were significant differences in fibration degrees between Rg1,Rb1 groups and control group ( P <0.01).Serological testing showed that Rg 1 and Rb1 remarkably reduced the levels of ALT ,PCⅢin serum,enhanced SOD activity and decreased MDA levels .Rg 1 and Rb1 also significantly increased the activities of respiratory chain complex Ⅰ,Ⅲ,Ⅳ and total ATPase ( P <0.01). Conclusion Panax notoginseng saponins-Rg1,Rb1 has inhibitory effect on hepatic fibrosis through protecting the mitochondria of rats with liver fibrosis .