Influence of antibiotic paintcoat of Ag-containing cobalt chromium alloy on expression of Bcl-2 and Bax protein of L929 cells
Abstract：Objective To investigate effect of antibiotic paintcoat of Ag-containing cobalt chromium alloy on L929 cells in order to explore its biological effect .Methods The plasma spraying technique was used to prepare 10 pieces of Ag-containing cobalt chromium alloy antibiotic paintcoats ( 10mm ×10mm ×1mm ) as experimental group,and prepare 10 pieces of cobalt chromium alloy (10mm ×10mm ×1mm) as control group. Leaching liquor of two kinds of alloys was prepared .The immunohistochemistry-SABC method was adopted to detect their effects on expression of Bcl-2 and Bax protein of L929 cell, and semi-quantitative analysis for the protein expression intensity was performed in both groups by image pro plus 6.0 in both groups .Results The expression of Bcl-2 and Bax in L929 cell was weakly reactive in cells cytoplasm in Co Cr/Ag alloy group.The ratio of Bcl-2/Bax was 1.239 ±0.046 in Co Cr/Ag alloy group and 1.244 ±0.086 in Co-Cr group,respectively,there was no significant difference between the two groups ( P >0.05).Conclusion There is no significant difference in expression of Bcl-2 and Bax protein of L 929 cells between Ag-containing cobalt chromium alloy antibiotic paintcoat and routine cobalt-chromium alloys .
Keywords：bcl-2immunohistochemistrycobalt chromium alloysAg-containing antibiotic paintcoat
Publication Date：2013-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 2728-2730 )