Comparison between lymphocyte multi-gene differential expression and lymphocyte surface protein molecule expression phase in rats undergoing heart transplantation
Abstract：Objective To establish the animal models of variant heterotopic cardiac transplantation in rats in order to observe the effects of lymphocyte multi-gene expression and CD4,CD8 molecule expression as well as cyclosporin A before and after heart transplantation .Methods SD rats were used as donor and Wistar rats were used as recipient .The rats were divided into two groups, transplantation control group and cyclosporine A intervention group.The CD 4, CD8 molecule expression levels in peripheral blood lymphocyte were detected respectively at 24h, 72h,7d,10d, 12d before and after heart transplantation ,and the expression levels of lymphocyte coding gene were analyzed by fluorescence differential display .Results ( 1 ) Tansplantation control group: there were 7 genes differential expression , which were 24h earlier than CD4, CD8 molecular positive regulation expression.(2) Cyclosporine A intervention group:there were 5 genes differential expression in peripheral blood lymphocyte.(3) Cyclosporine A intervention group: 2.3-bisphosphoglycerate positive regulation expression was 24h later than that of control group,fuethermore,cyclosporine A could also inhibit gig18 gene transcription of peripheral blood lymphocyte within 7d after heart transplantation.Within 24h after heart transplantation,sodium channel protein gene was expressed positively in peripheral blood lymphocyte of cyclosporine A intervention group . Conclusion The comparison between lymphocyte multi-gene differential expression and lymphocyte surface protein molecule expression phase plays an important role in researching deeply the transplant rejection reaction mechanism and the action mechanism of cyclosporine A .
Keywords：heart transplantation modelratsperipheral blood lymphocytemulti-gene differential expression phaselymphocyte surface protein moleculeCD4CD8 molecule expression phase
Publication Date：2013-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 2725-2727,2728 )