Changes of peripheral lymphocyte subsets in rats with acute pancreatitis

doi:10.3969/j.issn.1002-7386.2013.17.004

Abstract：Objective To investigate the changes of immune functional status in the development of acute pancreatitis ( AP) by examining lymphocyte subsets of peripheral blood in rats with acute pancreatitis .Methods Sixty specific pathogen free ( SPF) male Wistar rats were randomly divided into five groups:blank group ( n =11),sham-operation group ( n =11),acute pancreatitis 6-hour group (AP 6h group, n =11),acute pancreatitis 12-hour group (AP 12h group, n =13),acute pancreatitis 24-hour group (AP 24h group, n =14).The animal models with acute pancreatitis wee established by injecting 5% Na-Tc into capsula pancreatic .The rats in blank group and sham-operation group were killed at 6 hour after the operation , however , the rats in the three acute pancreatitis groups were sacrificed respectively at 6,12 and 24 hours after the models were established .The content of amylase in serum and the counts of lymphocytes were detected by automatic biochemistry analyzer .The changes in the rats'pancreatic tissues were observed by Hematoxylin-Eosin ( HE) staining and were pathologically scored . Lymphocyte subsets (CD3,CD4,CD8,CD19,CD56) in peripheral blood were measured by flow cytometry .Results There were no significant differences in lymphocyte counts between sham-operation group and blank group ( P >0.05),however,with the development of course of disease the CD 3 +lymphocytes were gradually decreased ,but CD8 +lymphocytes were slightly increased , and the ratio of CD4 +/CD8 +was obviously decreased , however , the ratio of CD3 +/CD19 + was significantly increased .Conclusion The disorder of immune system is gradually aggravated with the development of AP .