Effect of Shenmai injection on electrophysiology of pacemaker cells in left ventricular outflow tract of rats with AMI
Abstract：Objective To observe the effect of Shenmai injection on electrophysiology of pacemaker cells in left ventricular outflow tract of rats with acute myocardial ischemia (AMI).Methods The rat models with AMI were established by ligation of anterior descending branch of coronary artery , then the rats were treated by peritoneal injectiong with Shenmai injection for 7 days.The conventional intracellular microelectrode technique was used to record action potentials of pacemaker cells in left ventricular outflow tract after Shenmai injection or physiological saline injection,respectively.The indexes included amplitude of action potential (APA),maximal rate of depolarization ( Vmax) ,velocity of diastolic depolarization of phase 4 ( VDD) ,rate of pacemaker firing ( RPF) , duration of 50% and 90% repolarization ( APD50 and APD90 ) .Results The VDD and RPF0 in 9% sodium chloride solution group were significantly decreased ,as compared with those in control group ,and APA and Vmax were also decreased,however,APD90 was obviously increased ( P <0.01).As compared with that in Shenmai injection group,Vmax in control group was significantly decreased ( P <0.05),however,there were no significant differences in VDD,RPF,APA between the two groups ( P >0.05).Conclusion The electrophysiological activityof pacemaker cells in left ventricular outflow tract of rats with AMI is significantly decreased ,however, Shenmai injection can improve cardiac function and prevent left ventricular remodeling in rats after AMI .
Keywords：acute myocardial ischemiaratsleft ventricular outflow tractpacemaker cellselectrophysiology
Publication Date：2013-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 2569-2570,2571 )