Analysis of Ultrasonic Synergistic Ionic Liquid Extraction Approaches for Polysaccharides from Rorippa indica and Their Antioxidant Functions
LIN Xun
Abstract：Using Rorippa indica(L.)Hiern as raw material,the extraction process of polysaccharide from Rorippa indica by ultrasonic-assisted ionic liquid and its antioxidant activity were studied by response surface methodology.Firstly,on the premise of single factor experiment,Box-Behnken experimental design and response surface method were used to optimize the four key factors,namely ultrasonic temperature,liquid-solid ratio,ultrasonic time and ionic liquid concentration,and analyze their effects on the extraction rate and antioxidant activity of Rorippa indica polysaccharide.The results demonstrated that the ideal processing parameters were ultrasonic temperature of 70℃,liquid-solid ratio of 26∶1,ultrasonic time of 30 min and ionic liquid concentration of 2.9%.Under these optimized conditions,three repeated experiments were carried out to verify the prediction accuracy of the model.The measured extraction yield of polysaccharide from Rorippa indica reached 76.44 mg/g,and the deviation was only 0.12%compared with the predicted value of the model,which proved the high reliability and practicability of the regression model.The polysaccharide from Rorippa indica showed remarkable scavenging effect on DPPH and OH free radical,and the half-inhibitory concentration(IC50)values were 61.91 mg/L and 134.98 mg/L,respectively,which indicated that the polysaccharide from Rorippa indica had good antioxidant activity under the optimized technological conditions.The research offered a theoretical foundation for the in-depth development and use of Rorippa indica,and also provided a potential possibility for its application in functional food and medicine.
Keywords：ultrasound-assistedionic liquidRorippa indicapolysaccharideresponse surface methodologyantioxidation
Publication Date：2026-03-20
Online Publishing Date：2026-03-26(First online date of this platform, not the publication date of the document)
Pages：7( 22-28 )