Study on Extraction of Polyphenols from Oenanthe javanica by Ultrasonic-assisted Ionic Liquid and its Antioxidant Activity

CHEN Wenjuan; CHEN Jianfu; ZHUANG Yuanhong

doi:10.13501/j.cnki.42-1908/n.2025.12.015

Abstract：To extract polyphenols from Oenanthe javanica efficiently and explore its antioxidant activity,ultrasonic-assisted ionic liquid method was used,and the effects of ultrasonic time,ionic liquid concentration,ultrasonic temperature and liquid-material ratio on the extraction yield were investigated by single factor experiments.The extraction process was further optimized by Box-Behnken response surface design,and the antioxidant activity of the extract was evaluated by DPPH·,ABTS+·,·OH and O2-·scavenging experiments.The results showed that the optimum extraction process was ultrasonic time of 20 min,ionic liquid concentration of 0.31 mol/L,ultrasonic temperature of 62℃and liquid-material ratio of 31∶1.The extraction yield of polyphenols reached 56.91mg/g,and the relative error with the predicted value of the model was 0.65%,which verified the prediction efficiency and reliability of the mathematical model.The antioxidant activity experiment showed that the scavenging effect of Oenanthe javanica polyphenols on 4 kinds of free radicals was concentration-dependent,among which the scavenging effect on ABTS+·was the strongest,and its median inhibition concentration(IC50)was only 53.19mg/L,while its scavenging effect on O2-·free radicals was relatively weak,with IC50 reaching 302.78 mg/L.At the appropriate concentration,the scavenging rates of 4 free radicals were all above 72%,especially for ABTS+·,which could reach 92.67%of vitamin C.The research showed that the ultrasonic-assisted ionic liquid method was a feasible technology for efficient extraction of polyphenols from Oenanthe javanica,and the polyphenols from Oenanthe javanica had broad-spectrum and efficient antioxidant activity,which provided scientific support for its functional development and utilization.