Selenium Uptake and Assimilation and Their Effects on Adventitious Root and Bulblet Regeneration in Selenium-enriched Scale Cuttings of Lilium lancifolium
DUAN Yuqing
LUO Yuan
YANG Yuying
ZHANG Meide
DENG Zhijun
Abstract：To investigate the mechanisms of selenium metabolism and regeneration in Lilium lancifolium Thunb.scales under selenium-enriched cuttage conditions,scales were treated with sodium selenate at concentrations of 0,3,6,12,and 18mg/kg in a composite substrate for 40 days.Morphological regeneration indices,selenium content,jasmonic acid(JA)and malondialdehyde(MDA)levels,activities of peroxidase(POD)and catalase(CAT),as well as the expression of genes involved in selenium uptake and assimilation(LlSULTR1;1,LlAPS)and JA synthesis(LlAOC,LlLOX)were measured.The results indicated that both total and organic selenium content in the scales increased with rising sodium selenate concentration.The highest organic selenium proportion of(53.2±2.3)%was observed at 6 mg/kg.This treatment also resulted in peak JA content,maximal POD and CAT activities,minimal MDA content,and significantly upregulated expression of the aforementioned genes.Meanwhile,the rooting rate and bulblet induction rate were significantly enhanced,reaching(65.6±5.1)%and(86.3±5.5)%,respectively.However,when sodium selenate exceeded 6mg/kg,the organic selenium ratio decreased,JA synthesis was inhibited,antioxidant capacity declined,and adventitious root formation was significantly suppressed.In conclusion,6mg/kg sodium selenate was the optimal concentration for selenium-enriched cuttage culture of L.lancifolium scales.These findings provided a theoretical basis for improving selenium-enriched cultivation and cutting propagation techniques for L.lancifolium.
Keywords：Lilium lancifolium scalessodium selenateselenium uptake and assimilationjasmonic acidantioxidant defenseregeneration response
Publication Date：2025-12-30
Online Publishing Date：2025-12-10(First online date of this platform, not the publication date of the document)
Pages：7( 483-489 )