Metabolomics Combined with Network Pharmacology Reveals the Anti-T2DM Mechanism of Polygonatum kingianum var.grandifolium
LAN Demiao
YAO Jinkun
HUANG Jie
WEI Zhiyu
SHI Guangdi
XIAO Qiang
Abstract：In order to gain insight into the potential therapeutic effects of nucleotides and their derivatives from Polygonatum kingianum Var.grandifolium rhizomes on type 2 diabetes mellitus(T2DM),broadly targeted metabolomics,network pharmacology and molecular docking were used for analysis.The intersecting targets of key active components in traditional Chinese medicine for the treatment of T2DM were obtained through widely targeted metabolomics and relevant databases.Subsequently,enrichment analysis was performed on the intersecting targets.A ″component-target-pathway″ network model was constructed,and molecular docking was conducted.The results showed that the key active ingredient of nucleotides and their derivatives in Polygonatum kingianum Var.grandifolium rhizomes for the treatment of T2DM may be Adenosine,Cyclic 3',5'-Adenylic acid and Uridine 5'-diphosphate;The core targets may be AKT1,RAC1,HSPA8 and P2RY1;Signaling pathways may include the Rap1 signaling pathway,nucleotide metabolism,lysine degradation,and purine metabolism.The molecular docking results showed that the nucleotides and their derivatives in Polygonatum kingianum Var.grandifolium rhizomes had good binding activity to the target.This study demonstrated that nucleotides and their derivatives in Polygonatum kingianum Var.grandifolium rhizomes had multi-components,multi-targets and multi-pathways for treating T2DM.It provided a certain theoretical basis for T2DM drug development.
Keywords：metabolomicsnetwork pharmacologyPolygonatum kingianum Var.grandifoliumnucleotides and their derivativestype 2 diabetes mellitusmolecular docking
Publication Date：2025-06-20
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：8( 174-181 )