Transcriptome Transcriptome and Metabolome Analysis to Reveal Saponin Biosynthesis of Polygonatum Kingianum Var.Grandifolium
CUI Hongshi
LIANG Siqi
SHI Guangdi
CUI Lingjun
XIAO Qiang
Abstract：In order to enrich the transcriptome data of the rhizome development of Polygonum kingianum var.grandifolium,and explore the functional genes involved in saponin biosynthesis,based on Illumina deep sequencing platform,this study conducted transcriptome sequencing on rhizomes of Polygonum kingianum var.grandifolium at seedling stage,and further analyzed the biosynthetic pathway of saponins in secondary metabolic pathway.The results showed that a total of 198 296 transcripts and 92858 unigene sequences were obtained from the transcriptome data of rhizomes of Polygonum kingianum var.grandifolium by splicing,and 238 significantly different metabolites were detected based on the ultra performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS)detection platform and the self built database.According to the injection and differential gene expression of enzyme genes in the saponin synthesis pathway of Polygonum kingianum var.grandifolium and the enrichment analysis of metabolism pathway,three related pathways and enzyme genes in the saponin synthesis pathway were found,as well as the 33 enzyme-genes,and it was speculated that these enzyme genes played a crucial role in the saponin synthesis pathway.This study provides a wealth of reference data for the study of transcriptome and metabolomics of Polygonum kingianum var.grandifolium,and is of great significance for the study of functional genomics of Polygonum kingianum var.grandifolium.
Keywords：Polygonatum kingianum var.grandifoliumtranscriptomicsmetabolomicsIllumina deep sequencing platformultra performance liquid chromatographysaponin biosynthetic pathway
Publication Date：2024-03-20
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：10( 1-10 )