Rapid Determination of Three Sweeteners Content in Liquor by UltrahighPressure Liquid Chromatography-tandem Mass Spectrometry
HONG Jian
ZHOU Dazhai
TANG Qiaoyu
Abstract：A rapid and sensitive quantitative analysis method for three sweeteners (acesulfame,saccharin sodium and sodium cyclamate) in liquor samples by ultra-performance liquid chromatography-time of flight mass spectrometry (UPLC-TOF-MS) was established.After the sample was diluted by ultrapure water,a 0.1% formic acid water-methanol solution was used as the mobile phase and subjected to isocratic elution at Endeavorsil C18 (available from DiKMA,1.8μm,100mm×2.1mm),and the three kinds of sweeteners were determined by quantitative analysis by electrospray ionization source ESI negative ion mode.The experimental results show that under the optimal conditions,three sweeteners achieve full separation within 7 min.The detection limit (LOD,S/N=3) was 1.0~8.0μg /mL,(LOQ,S/N=10) was 3.33~26.7μg/L.There was a good linearity between the three sweeteners in the range of 1 ~ 320μg / L,the correlation coefficient was over 0.998.the recoveries in the liquor samples of compound liquor were in the range of 96.33%~104.16%,RSD were in the range of 0.17%~1.36%;the recoveries in the liquor samples of flavor types liquor were in the range of 92.38%~100.36%,RSD were in the range of 0.98%~1.85%;the recoveries in the liquor samples of fruit liquor were in the range of 99.97%~103.88%,RSD were in the range of 0.52%~1.59%.The method is sensitive,adaptable and reproducible to the rapid determination of sweeteners in liquor samples.
Keywords：UPLC-TOF-MSliquorsweetener
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 162-166 )