Comparison of Gene Amplification Effect under Three Methods of PCR Based on Degenerate Primers
LI Zhiqiang
HE De
LI Cuixin
Abstract：Three PCR methods(conventional PCR, touchdown PCR, nested PCR) were used to amplify the DNA topoisomeraseⅡfragments of Pleurotus ostreatus and P.eryngii var ferulae with degenerate prim-ers,which were designed with the gene sequences of DNA topoisomerase Ⅱfrom NCBI.Their PCR sensi-tivity and specificity were compared .The result showed that the sensitivity and specificity of nested PCR were highest , and its sensitivity was 100 fold higher than conventional PCR , which indicated that the nested PCR was more suitable for PCR amplification under degenerate primers .This study will have some guidance significance to obtain the specific gene through degenerate primers .
Keywords：degenerate primerconventional PCRtouchdown PCRnested PCR
Publication Date：2015-04-02
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 170-173 )