Study on the Correlation between Content of Artemisinin and Density of Glandular Trichemes of Artemisia annua L
MOU Di
HUANG Rong
XIAO Kailun
PU Guijin
ZHOU Dazhai
QIN Daji
XIAO Qiang
ZHOU Yifeng
Abstract:In order to study the content of artemisinin and the density of glandular trichomes,the fluores-cence microscope and scanning electron microscopy ( sem) are used to determinate 8 groups of different artemisinin yield.At the same time,the artemisinin content was determined by HPLC method,then SPSS software is used to study correlation between them.The results show that:①the density of glandular tri-chomes is different for different leaves, the maximum mean value observed through fluorescence micro-scope and scanning electron microscopy (sem) was No.4 plant,the average density was (75.7±16.0)a/cm2 ,respectively,and (29.4±5.9) a/cm2 ,which proves fluorescence microscope is more suitable for the determination of the density of glandular trichemes,and scanning electron microscopy ( sem) is better sui-ted to observe the structure of the glandular trichomes;②there is a significant linear correlation relation-ship the density of glandular trichomes and the content of artemisinin,the correlation index is 0.734,P=0.038<0.05.It proves that artemisinin is mainly producted by glandular trichemes,and the associated sig-nificance does not reach extremely significant level, which means a small amount of artemisinin is pro-duced in other types of cells.
Keywords:Artemisia annua LGlandular Trichemesartemisininstereo fluorescence microscopescan-ning electron microscope
Publication Date:2014-01-01
Online Publishing Date:2025-08-15(First online date of this platform, not the publication date of the document)
Pages:5( 262-265,317 )