Comparative Study on the Different Organization of Genomic DNA Extraction in Pinus armandi Franch
XIE Dongmei
CHEN Guoming
HE De
LI Cuixin
ZHANG Xincong
Abstract：It is the critical first step how to obtain high-quality DNA in molecular biology research.In this paper, their genomic DNA from stem,leaves and endosperm of Pinus armandi Franch were extracted using five methods, which were the classical CTAB method, modified CTAB method, simple CTAB meth-od, SDS micro-extraction method and Zigenhagen method.Then, the extracted DNAs were analyzed by ultraviolet spectrophotometer, agarose gel electrophoresis and standard deviation and standard error of a-rithmetic mean.The results showed:①The modified CTAB method was appropriate for leaves genomic DNA extraction, whose yield was moderate, changes of standard deviation and standard error were lower, and DNA quality was better and stable;②In stem genomic DNA extraction, the yield and the purity of DNA from the Zigenhagen method were highest and its changes of standard deviation and standard error were lowest.Its DNA quality was obviously better and stable;③For endosperm, the yield and the purity of DNA from simple CTAB method was highest, and its changes of standard deviation and standard error were lowest, which indicated that the DNA quality from simple CTAB method was obviously better and stable.Therefore,different methods should be used to extract genomic DNA in the different tissue of one and the same plant.To obtain the best result, the method of DNA extraction need to be explored in prac-tice.
Keywords：Pinus armandi Franchclassical CTABmodified CTABsimple CTABSDS Micro-Extrac-tionZigenhagen
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：7( 255-261 )