Determination of Selenocystine and Selenomethionine by UPLC-TOF-MS
XIAO Qiang
QIN Yan
Abstract：The analysis of selenocystine( SeCys) and selenomethionine( SeMet) by UPLC-TOF-MS was studied.The separation was performed on a ZORBAX 2.1×50 mm 1.8 Micron C-18 column with methanol 0.1%( formic acid ∶water) ( with gradient) as mobile phase at a flow-rate of 0.2 mL/min,and the col-umn temperature of 25℃.TOF Mass spectrometry was used as the detector, and EIC mode was selected. The linear regression curve of SeMet and SeCys were 18.304~45.76ng,14.562~38.832 ng,respectively, with R2 being 0.999 0,0.999 6 and RSD being less more than 1.5%.SeMet and SeCys could be rapidly and accurately quantitative determined within 3 minutes.
Keywords：selenium compoundschromatographyhigh pressure liquidelectrospray ionization
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 245-247 )