A Study on Subculturing of Schoenorchis fragrans
Abstract：The subculturing of protocorm like bodies （plbs） on Schoenorchis fragrans was studies. An available method of plbs subcuhuring would be found to benefit the whole process of Schoenorchis fragrans tissue culture, confirm the possible concentrations of cytokinin and auxin for the plbs subculturing, Cyto- kinin 6-Benzyladenine （6-BA） and Auxin Napthalene Acetic Acid （NAA） was required in the nutrient medium. Stimulated multiple shoot formation. On MS medium （ 1949）, the axillary buds and apical buds obtained from the young shoots of Schoenorchisfragrans formed plbs in 6 to 10 weeks. This experiment al- so would carry out to test the efficiency of different concentrations of cytokinin （6-BA and NAA） on plbs subcuhuring of tissue culture in Schoenorchis fragrans. The best treatment is the concentrations of cytokinin 6-BA 2.0mg·L^-1 and NAA 0.5 mg·L^-1. 6-BA has the benefit for plbs multiplication, and NAA was good at young seedling formation. Coconut milk has special effect on plbs multiplication and young seeding formation. For plbs multiplication, the best concentration of cytokinin is 6-BA 2.0 mg·L^-1 and NAA 0.5 mg·L^-1. For young seedling formation, the best concentration of cytokinin is that 6-BA 2.0 mg·L^-1 , and NAA 1.0mg·L^-1. Minitabl4.12 statistical analysis software was used for ANOVA. The results indicated that the main effects and interactions effects of both factors （NAA and 6-BA） on plbs multiplication were all significant.
Keywords：Schoenorchis fagranstissue culturesubculturingplbsmultiplication
Publication Date：2012-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 150-152 )