Choice of HPLC Methods of Extracting CPT from the Tender Leaves of Camptotheca acuminate Decne
Abstract：A method to extract the CPT from the tender leaves of Camptotheca acuminate Decne through HPLC. Using waters symmetry C18 （ 150min×3.9mm,5μm） HPLC. Acetonitrile ： water = 35：65 （V/V） as the mobile phase, 1.0mL/min flow rate, detection wavelength 254nm, column temperature ：25 ℃, sampling quantity 10μl. With different degrees of methanol extraction with different concentrations of alkali method comparison. Conclusion：the best method is to choose methanol as the solvent at 80% to 50 degrees for 1 hours under ultrasonic extraction.
Keywords：Camptotheca acuminataHPLCextraction
Publication Date：2012-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 139-141 )