High Density Fermentation of Lactobacillus paracasei F50 Enhances Aflatoxin Removal and Associated Non-targeted Metabolomics
ZHANG Xinrui
YANG Gang
LI Aijun
PENG Xiaowei
KAN Jianquan
Abstract：Lactobacillus paracasei plays a significant role in the food industry and safety.To save equipment space,reduce production costs,and enhance application value,factories conduct high-density fermentation during production.Low-cost molasses and defatted Idesia polycarpa Maxim were used as substrates for the high-density fermentation of L.paracasei F50.The culture conditions and medium components were meticulously optimized,with strain density serving as the primary response variable.The results showed that the optimal fermentation conditions for L.paracasei were a 3.5%inoculum,culture pH of 6.7,1.7%calcium carbonate,and 3.2 g/L defatted Idesia polycarpa meal.The final strain density increased from 7.3×108 CFU/mL to 1.7×1010 CFU/mL,approximately 23 times that of the conventional culture.The aflatoxin removal rate increased from 18.15%before optimization to 48.17%after optimization.To investigate the reasons for the improved aflatoxin removal rate after high-density cultivation,liquid chromatography-mass spectrometry(LC-MS)was used to analyze the supernatant of L.paracasei before and after optimized cultivation using non-targeted metabolomic techniques.The results showed that there were 535 main differential metabolites in the fermentation broth of L.paracasei before and after optimization,with increased levels of lipid,polypeptide,and organic acid metabolites.Simultaneously,the glutamate metabolic pathway was enhanced,promoting glutathione biosynthesis.This study provides a reference for high-density fermentation and biological detoxification research on L.paracasei.
Keywords：Lactobacillus paracaseihigh density fermentationdefatted Idesia polycarpanon-targeted metabolomicsaflatoxin
Publication Date：2026-01-20
Online Publishing Date：2026-03-31(First online date of this platform, not the publication date of the document)
Pages：12( 41-52 )